The method of saving of the bacteria includes the oblique low temperature preservation method, the liquid paraffin coverage method, the sand preservation method, the filter paper preservation method, the glycerin pipe preservation method, and the freezing drying method.1. Save at low temperature: Inoculate the bacteria to the appropriate solid oblique medium, wrap the cotton with oil paper, and then transfer it to 2-8 ° C and save it in the refrigerator.2. Lock paraffin coverage method: Use liquid paraffin to cover the oblique bacteria to isolate it from air.
1问苗哈国际交易中心. What are the ways to save bacteria?
（1）将细菌接种到适当的固体斜率培养基中。细菌完全生长后，带有油纸的棉，然后将其转移到2-8°C，然后将其存储在冰箱中。
（2）如果是真菌，螺纹细菌和孢子，通常可以将其储存约2-4个月，并且有必要将其移植一次。如果是酵母，则通常可以存储约2个月
（1）将液体石蜡放入三角瓶中，用棉塞，用牛皮纸绷带，然后在0.1mpa，121.3°C下灭菌约30分钟，然后将40分钟°C蒸发并蒸发到热力中盒子。
通常，可以保存无孢子的细菌1年，脑膜炎可以保存约3个月（37）℃环境环境）本质
（1）准备河沙，加入10％稀盐酸，然后加热并煮沸约30分钟，去除内部有机物，倒出酸水，将其冲洗至pH中性，然后干燥，筛（40测量筛子）
（2）制备非凹入的植入物非文化层薄层或红色土壤，浸泡并清洗几次，直到pH中性，然后干燥，碎，筛分（100筛网）以去除粗颗粒。
(4) Cultivate bacteria in the oblique medium, obtain a strong bacteria, and then use sterilized water to clean the cells or spores in the 3-5 injection of 3-5 into the inclined culture.
(5) Absorb the bacteria suspension with a sterilized straw, and then evenly drip into the sand tube (0.2-0.5ml/tube).If it is a thread or mold, the spore can be picked directly into the sand tube.
(6) A vacuum absorption of water in the sand tube.
(7) Mel time with the flames, and then store it in a dry place at a low temperature (4-6 ° C), or wrap sand with cowhide or plastic paper and place them in a dryer.During the preservation, the survival of the bacteria is checked every 6 months.哈国际交易长沙
4. Filter paper preservation method
(1) Prepare the filter note (0) .5 × 1.2cm), the pipe (0.6 × 8cm), and then place 1-2 filter strips per sacred pipe, plug it with a cotton, 0.1MPa, destroy 121 ℃, destroy 121 ℃The bacteria are about 30 minutes.
(2) Treatment of bacteria at the appropriate slope medium to obtain a strong bacteria.
(3) Add 1-2ml sterilized and skimmed milk to the sterilized dine, take the moss of the loop, mix uniformly in the milk, make a thick suspension, and then remove the paper strip from the tube of the tunnel and soak it inIn the bacterial suspension, absorb it, put it back to the pipe, and plug on the cotton.哈国际交易正规的吗
(4) Put the pipe tube into the dryer (containing five phosphorus phosphorus as a water absorption agent), and use the vacuum pump to get gas to dry.
(5) Put the cotton into the tube, melt it with flames, and then store it in a low temperature environment.
5. Glycerin pipe preservation method
(1) Prepare 80%glycerin and then sterilize high -pressure steam.
(2) Cultivate bacteria at the appropriate slash medium, and use sterile water to make a high concentration of bacteria suspension.
(3) Take out 1ml glycerin and bacterial fluid and mix well, so that the concentration of glycerin is about 10-30%, and then stored in the environment at minus 70%° C.
6. Frozen and dry preservation method
The cells quickly frozen at low temperature, and then dried under vacuum conditions to stop the growth of microorganisms and enzyme activity.At the same time, in order to ensure that cells are not damaged during the process of freezing and water sublimation, the protective agent should be used to prepare cell suspension (the soluble that protects the effect during freezing and dehydration), and the hydrogen bonds and ion bonds areFate stabilize the shape of the cell component.
Second, two, bacterial operation rules for generations
Lift the alcohol lamp and burn the inoculation ring above the flame.
2. Use the left thumb, index finger, and middle finger to clamp the original bacteria inclined medium (bacterial tube) and the new sloping medium to be vaccinated.The fungus is tube front, the inoculation tube is taken, and then the test tube (45 °) is oblique to allow the test tube to obliquely upward, and the opening of the two test tubes is flat.
3. Rotate the two tubes next to the flames, and then hold the handle of the inoculation ring with the right hand, and burn the inoculation ring vertically on the flame.The ring on the top must be red, and the part of the test tube when vaccination should be burned through the flame.
4. Use the little finger and palm of your right hand, as well as the unknown and little finger, pull out the cotton plugs with two tubes, hold it, and then pass the test tube on the flame.
5. Insert the inoculation ring into the bacterial pipe and contact the medium that exposes the growth of sterile moss.After cooling, select a little moss from the slope, then quickly insert the inoculation tube, and gently draw the line on the slope (from the bottom to the top to the top to the top).
6. After the vaccination, the mouth is sterilized through the flame, and the cotton plugs.
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